The Fractionation of Rye Diffusate by Continuous Paper Electrophoresis and the Effect of each Fraction on the Flowering of Annual Meadow Grass.

Abstract

Several substances contained in the rye diffusate have been continously collected by the method of continous paper electrophoresis. The seeds of annual meadow grass (Poa annua L.) were sown on the top of agar block partially soaked in each fraction, adjusting the pH from 3.5 to ca. 6. The seedlings grown on the agar block were transplanted into the plastic pot later, and were placed under the long day condition. The plants treated with the fractions of 2∼3 and 6∼7 flowered earlier than the control. The number of leaf were also few in those plants. Almost similar results were obtained in this experiment, comparing with the data reported previously.