A comparison was performed between the theoretical values expressing the degree of the injury calculated from the experimental results and the observed values of that of wheat plants which suffered the chilling injury at the region of Shigarami. 1) The theoretical values were calculated from two numerical data the degree of the injury per every heading date obtained from the artificial low temperature treatment and also from the investigated results of heading status in the fields. 2) It was found that the theoretical values and the observed values agreed considerably, when the two values were applied to the experiments on the seeding period at the region of Shigarami. 3) This fact is thought to indicate that the quantitative estimation of the injury by the chilling may be possible.