Studies on Resistance of Wheat Varieties to Scab (Gibberella zeae (Schw.) Petch.). : V. The improved method of inoculation to leaf blade.

Abstract

In this study, an attempt was made to find a more simple method of inoculating scab onto leaf blade, because of the inconvenience and the troublesomeness of the previous method including fixation of vinyl ring containing potato agar medium on the leaf blade to be inoculated. It was found that the use of small pieces of wheat or spinach leaves gave a more satisf factory result than the use of potato agar medium. The procedures used were as follows: 1. Small pieces of each 8 mm in diameter are punched from wheat or spinach leaves and soaked in boiling water for 3 minutes. 2. Three layers of the boiled leaf pieces are placed on the region of leaf blade to be inoculated, and adhered with cellotape followed by wrapping with wrap-film. 3. The treated plants are kept at 20∼25°C. Moreover, the comparison of wheat and spinach leaved indicates that spinach is superior to wheat leaf as the inoculating medium, and results in the more severe and more rapid infection of scab.