Abstract
In the course of an investigation to know the reliable estimate of resistance of wheat varieties to scab, it was found that a given floret of a wheat head could be successfully inoculated by the improved method in the out-or indoor experiment. The improved method of inoculation was done as follows; 1. Clip off the wan from all of the spikelets of a wheat head. 2. Remove the immature grain from the floret to be inoculated. 3. Two little holes were pricked with a pin in the outer glume and inoculate with scac. 4. Put a small amount of boiled spinach leaves, as nutrient of scab, between the inner and outer glumes of the inoculated floret. 5. Wrap the treated head with wrap film. 6. Keep the plant thus treated at 20∼25°C. This improved method was expected to be of use for the systematic and reliable comparison among the varietal responses to scab. The reason was that the scab lesion, if only the terminal spikelet was inoculated, was assumed to spread downward to various distance depending upon the varietal responses. But the degree of lesion from the artificial inoculation did not always coincide with the degree of damage received in the field by unknown reason, which must be clarified by the future works.
