On the Culture of the Rice Seminal Roots with Scutella in vitro.

Abstract

The seminal roots with various tissues of the caryopsis, whole seminal roots and 1cm long seminal root tips of rice, variety Noorin No. 29, were cultured with several kinds of media. On the medium containing only White's inorganic salts plus 6.0 percent sucrose, the seminal roots with scutella (Fig. 1, ii) showed very good growth (about 50-60 cm in root length). On the other hand, the seminal roots with scutella were cultured by adopting the manner of feeding sucrose from the side of scutellum in small vial and inorganic salts through the root in flask (Fig. 2). In these cultures especially in the case of A type culture, it was found that the seminal roots grew more than 100 cm (with the maximum of 152 cm in the case of A type culture), when the concentration of sucrose was raised to 12.0 percent. These results suggest that culture of the seminal root with scutellum by using A type culture is a useful method for studies on the physiology and morphogenesis of rice roots.