Lipid Granules in Free Cells in Suspension Culture of Rice

Abstract

A number of single cells and cell aggregates were produced from rice callus tissues in liquid medium at 25-30°C. The freely suspended living cells and aggregated cells were containing one or more of spherical granules, 2.8μ of average diameter. The granules disappeared with acetone, ethanol, ethylene glycol, formalin, isopropanol, pyridine, triethyl phosphate and chloroform methanol mixture. They were also stained blue by 1% or 0.02% Nile blue. But the colour was not removed by 5% acetic acid or 0.5% hydrochloric acid. From these results, it is revealed that the granules are composed of liquid, acidic lipids.