Histochemical Studies on Accumulating Process of Fat and Aleurone Grains in Rice Kerne

Abstract

This is an histochemical study of fat and aleurone grains in rice kernel during ripening Processes. The results obtained are summarized as follows: 1) It was observed that the aleurone layer and embryo was rich in fat by staining with Sudan black B and Sudan III. Epidermis of nucellus also showed black coloration with Sudan black B as its cell wall thickened. 2) Fat accumulation in aleurone layer began at the 7th day, and was completed at the 15th day after flowering respectively. 3) Aleurone gsains were clearly observed by ether treatment for removal of fat in cross section of rice kernel. 4) Aleurone grain of rice kernel was a globle, about 2μin diameter and simpld with no structure in it. 5) This aleurone grain was stained by fuchsin basic and carmine acetate, but not by safranine, methylen blue, methyle orange, eosin, neutral red and iodine potassium iodine 6) Protein reaction of aleurone grain by ninhydrin, Millon's reagent and mercuric chloride BPB was not observed. 7) This grain is soluble in 0.1 N-HC1, but insoluble in 0, 1 N-NaOH. In view of the fact that 0, 1N-HC1 is the reagent of extraction of phytine in cereal grain, aleusone grain of rice kernel seems to be rich in phytine. 8) It was late ripening period (the 20th day after flowering) that aleurone grain was clearly noticed in aleurone layer of rice kernel.