A Light and Scanning Electron Microscopic Study of the Initiation and Development of Sugarcane Callus

Abstract

Initial callus was primarily formed on the cut ends and occasionally on the abaxial surface of sugarcane immature leaf explants. The growing callus became nodular and purple in colour. Some specimens became white. Subsequent growth of callus led to the breaking through callus surface. The newly formed cells emerged from the breakages and grew into oval and hairy, elongated shapes ; this led to the formation of soft callus masses on the explants where spherical structures developed consisting of meristematic cells.