Isolation and Culture of Protoplast from Finger Millet (Eleusine coracana) Callus

Abstract

High yield of protoplast was obtained from the green callus tissue of finger millet at one week after subculture on MS medium with 0.2 mgl^-1 2, 4-D and 0.1 mgl^-1 kinetin. The protoplasts were released using the enzyme solution containing 2% Cellulase YC and 0.2% Pectolyase Y-23. About 75% of the protoplasts cultured in the MS liquid medium with 0.4 mgl^-1 2, 4-D and 0.1 mgl^-1 kinetin supplemented with 5% coconut water were started to show regeneration of the cell wall within 12 h. Cell division was observed within 24 h after culture. The protoplasts that underwent division were about 15%. The sustained cell division could lead to microcolony formation in 4 d after culture. However, further growth did not occur and viability gradually decreased during culture.