Abstract
High yields of viable protoplasts were obtained from leaf mesophyll of 10-day-old peanut seedlings (Arachis hypogaea L. var. Lokal). An enzyme solution containing 1% Cellulase YC, 2% Meicelase P-1, 0.15% Pectolyase Y-23 and 0.5% Macerozyme R-10 was found to be the best for protoplast isolation. The protoplasts were then cultured in liquid or in agarose-gel modified KM8P medium. Cultured protoplasts divided 2 days after culture. In solidified media, protoplasts divided continually and formed many colonies 1 month after culture. The best viability and colony formation was obtained in a culture medium solidified with 1.2% agarose.
