Abstract
This study examined the effect of MFP/BS combination dentifrice (MFP containing bamboo salt that has various mineral components) on fluoride uptake and acid resistance of bovine enamel. The experiment utilized bovine enamel which was treated with 5 different groups of dentifrices for 24 hours : 1) C : control dentifrice containing abrasive only, 2) F : MFP dentifrice, 3) FB : MFP/BS dentifrice, 4) FBV : MFP/BS/VE (vitamin-E) dentifrice, and 5) FSV : MFP/TS (table salt)/VE dentifrice. Fluoride uptake and acid resistance of bovine enamel treated with dentifrices were assessed by chemical analysis and morphological observation was carried out using SEM (scanning electron microscopy) , XRD (X-ray diffractometer) and XMA (X-ray micro analyzer). In order to assess fluoride uptake, each of the four dentifrices was compared with the control dentifrice as to the amount of fluoride uptake. The first layer (3〜4μm depth) of the enamel surface showed significant fluoride uptake in the experimental groups (F, FSV, FB, FBV) contained MFP comparing the control (p<0.05). In the third layer (appr. 17〜20pm depth) only the FB and FBV groups showed significant fluoride uptake compared to the control (p<0.05). In acid resistance test of the treated enamel, the F group showed inhibition of Ca dissolution for 6 hr, and the FB and FBV groups for 12 hrs (p<0.05). In SEM observations, the surface of the FB and FBV treated enamel showed innumerable adhesive particles on the enamel surface, which was analyzed by XRD and found to be hydroxyapatite and precursors of hydroxyapafite. In the FB and FBV treated enamel, K_5P_3O_<10>, KCa(PO_3)_3 and Ca_4O(PO_4)_2, products were detected. The ratio of Ca/P in the enamel surface calculated using XMA, was 1.62 for FB and 1.63 for FBV, which is close to 1.67, the value of sound enamel. It is concluded that MFP/BS and MFP/BS/VE dentifrices are more effective in fluoride uptake and an acid resistance in vitro than the conventional MFP dentifrice and MFP/TS/VE dentifrice.
