Abstract
The author undertook the statistical investigation on the laboratory findings of tubercle bacilli from the patients' sputa in a general hospital having about eighty beds exclusive for tuberculosis patients in the suburbs of Tokyo. The period of this investigation was five years starting from August 1954 until July 1959, and the results obtained were the following.
For the smear examination, both modified Ziehl-Gabbet's staining method and the fluoro-microscopic method were employed. For the cultivation, preoperated by 8% NaOH, KH2PO4 Ogawa's media were introduced in this investigation.
The effect of airation by the rubber stopper with the small air passage and the ratio of contamination by the micro-organisms were also studied.
Generally speaking, the results of culture method were superior to that of smear method for the detection in the monthly clinico-bacteriological examination through the full periods with few exceptions.
The relation between the Gaffky's scale and the results of cultivation was found as follows.
The Gaffky's scale and the duration until the growth of bacillus was visible, related reversely, and the Gaff ky's scale and the number of colonies related directly. Those close numerical relations approved intensive correlation between smear method and culture method. Some new findings on the specimen of Gaffky's scale “O”was shown here.
The colony type of tubercle bacilli was substantially “Rough-type”, however the author identified 3 forms, and they were changeable within them even in the strain derived from the same sputum.
