Abstract
The purpose of this study was to examine the effect of sucrose upon the solubility of the dental hard tissues.
Enamel and dentine powder prepared from freshly extracted bovine teeth was suspended in the following 3 solutions, namely pH 7.5 tris-buffer solution (B), mol sucrose in solution B (S) and solution S autoclaved at 125°C for 1hr (CS). After 16 days incubation at 37°C, the amounts of Ca and P dissolved in each solution were determined. Solution S increased the solubility of Ca and P in dentine, but decreased it in enamel in comparison with solution B. Solution CS significantly increased the solubility of Ca and P in both enamel and dentine.
The free N-terminal groups in enamel and dentine incubated in solution B and solution CS for 16 days were determined by the use of Sanger's fluorodinitrobenzene method. No difference was found between the two solutions.
In tooth powder deprived of its organic components by extraction with ethylendiamine, in natural apatite powder and in lipid-extracted teeth powder, the solubility tests were carried out under similar experimental conditions.
The results indicated that the amounts of dissolved Ca and P were strongly influenced by the extraction of the lipid component.
In the caramelized sucrose solution, lactate, formate, acetate and pyruvate were found. Any one of these acids was able to decrease calcium ion concentration in slightly alkalized CaCl2 solution.
