Abstract
The effect of fluoride ion (NaF) on proliferation was examined in Vero cells derived from kidney tissue of Cercopithecus aethiops. Fluoride ion concentrations in the growth medium of 0.1, 1, 10, and 100 ppm were employed in the first experiment and of 0.1, 0.2, 0.5, 1, 2, 5, 10, 20, 50, and 100 ppm in the second. Simplified replicate cultures were prepared and cultivated for about five days. After cultivation, cell growth was estimated by enumerating cell nuclei concentration. A relationship between cell proliferation and fluoride concentrations in the growth medium was confirmed statistically and details from control and experimental cell growth were investigated.
Cell growth was accelerated by 17-18% with increasing fluoride ion concentrations of 1-10 ppm in first experiment. Maximum proliferation occurred at 2 ppm in the second experiment with a 22.6% rate of increase. Thereafter cell growth was reduced with increasing fluoride ion concentrations until near 100 ppm, at which concentration cell growth was completely stopped in both experiments.
