Antimicrobial effects of various electrolyzed water on root canal irrigation

Abstract

Abstract : Recently, the use of electrolyzed water has become widespread in a variety of fields. Although electrolyzed water have antimicrobial effects, they cause little harm to human being or the environment. We investigated antimicrobial effects of various electrolyzed water when used for root canal irrigation.

 The irrigants examined were two kinds of strong acid electrolyzed waters (SAW A and B), weak acid electrolyzed water (WAW), neutral electrolyzed water (NW), alkaline electrolyzed water (AW), 3%NaOCl and sterilized distilled water (DW). The root canals of extracted human teeth and artificial canals (epoxy resin) that had a working length of 10 mm and a size of #70 were used. Enterococcus faecalis was injected into the canal and then irrigated with 1, 3, 6, and 12 ml of each irrigant. After irrigation, the paper points used for sampling were incubated in tryptic soy broth at 37°C for 48 hours, and assessed for antimicrobial effects.

 All samples irrigated with NaOCl were negative growth (−). All DW samples were positive growth (+). For SAW, WAW and NW, some antimicrobial effects were seen on the artificial canals. However, there was a little effect on the extracted teeth. Antimicrobial effects of AW were not shown in artificial canal and extracted teeth.