Cytotoxicity of one-step adhesive resins on dental pulp cells and their effects on differentiation to odontoblasts

Abstract

Abstract : One-step adhesive resins have been widely used in restoration operations due to their simple procedure. However, the cytotoxicity of one-step adhesive resin is not fully understood. The purpose of this study was to investigate the effects of six one-step adhesive resins on cytotoxicity and differentiation to odontoblasts. Specimens from Absolute (AB), AQ Bond Plus (AQ), G-bond (GB), i-bond (IB), Adper Prompt L-Pop (APL) and Clearfil Tri-S Bond (S3) were eluted with fresh culture media for 24 hours. Human dental pulp cells and mouse odontoblast-like cells (MDPC-23) were cultured with extracts of the adhesive resins for 24 hours and cytotoxicity was judged by tetraxolium bromide reduction assay. AQ was the least toxic against both human dental pulp cells and MDPC-23 among the six one-step adhesive resins studied here. Furthermore, ALP activities in cell extracts of MDPC-23 cultured with each adhesive resin for 2, 4 and 6 days were measured to examine whether the resin affects the differentiation to odontoblasts. Only IB significantly inhibited ALP activity compared to control cells. In conclusion, AQ is the least toxic material to human dental pulp cells and MDPC-23 among the one-step adhesive resins examined in this study.