1996 Volume 6 Issue 2 Pages 211-215
A new type of umu test using chemiluminescence detection was developed. This method is made up of measurement of chemiluminescence which is generated from the glucose yielded as a enzyme reaction product of β-galactosidase, after several reaction steps. Salmonella typhimur-ium TA1535/pSK1002 and lactose was used as the tester strain and the substrate to measure β-galactosidase activity as an index of mutagenicity, respectively. This method was as sensitive as the fluorescence assay previously reported, and was also effective for detection of promutagens.
