Anticipated Mutation Assay Using Single-molecule Real-time (SMRT^TM) Sequencing Technology

Abstract

I describe here an anticipated mutation assay which estimates the frequency and spectrum of somatic point mutations in any genes of any samples by using SMRT^TM (Single Molecule Real Time) DNA sequencing technology, which will be released in 2010. The basic concept of the mutation assay is very simple: just prepare the target template and sequence it accurately. In this paper, I propose ideas on how to make the template and how to eliminate artifactual mutations caused by damage to the DNA template.