Abstract
I describe here an anticipated mutation assay which estimates the frequency and spectrum of somatic point mutations in any genes of any samples by using SMRTTM (Single Molecule Real Time) DNA sequencing technology, which will be released in 2010. The basic concept of the mutation assay is very simple: just prepare the target template and sequence it accurately. In this paper, I propose ideas on how to make the template and how to eliminate artifactual mutations caused by damage to the DNA template.
