Journal of Ecotechnology Research
Online ISSN : 1884-0388
Print ISSN : 1881-9982
ISSN-L : 1881-9982
Original Article
Ecophysiological analysis of halobacteria in bioaerosol
Teruya MAKIShinzi SUSUKIFumihisa KOBAYASHIMakiko KAKIKAWAMaromu YAMADATomomi HIGASHIChunsang HONGYutaka TOBOHiroshi HASEGAWAKazumasa UEDAYasunobu IWASAKA
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2008 Volume 13 Issue 4 Pages 309-313

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Abstract

The Asian dust phenomena, Kosa, have possibility to carry the microbial particles (Kosa bioaerosol) influencing the microbial habitats and the human health in Japan. In this study, the bioaerosol were collected at 600 m and 2 m above ground in Kanazawa city at the Kosa coming season, April in 2007. In the culture medium produced using seawater and lake water, the bioaerosol at 600 m indicated the microbial growth only in the seawater medium, but no growth in the lake water medium. The bacteria in bioaerosol at 2 m grew in both of the seawater and lake water media. The halophilic bacteria would maintain viable activities in the atmosphere. When the bioaerosol at 2 m was inoculated to the culture media including NaCl at some ranges of concentrations, the microbial growth was higher in this order of 0%, 3%, and 10% of NaCl concentrations, and no growth was detected in the 20% NaCl medium. According to the DGGE (denaturing gradient gel electrophoresis) analysis, same bacterial species would be included in the every NaCl concentration of culture medium, and other bacterial species could grow only in the culture medium with 3% or 10% NaCl concentrations. Therefore, the halophilic or halotolerant bacteria would survive in the aerosol at 2 m above ground, and may be related to the long-distant transfer of microbial population. The approach targeting the halobacteria will provide valuable information about the microbial transport across the ground-atmospheric area.

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© 2008 International Association of Ecotechnology Research
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