2000 Volume 11 Issue 4 Pages 99-105
In order to determine the exon-intron structure and the nucleic acid sequences of the equine genomic cytochrome P450 steroid 17α-hydroxylase/17,20-lyase gene (CYP17), inter-exonal PCR amplifications were performed with equine genomic DNA as a template. The amino acid coding region of the equine CYP17 gene was consisted of 8 exons and 7 introns, and the total 6217 base pairs of nucleotide sequence was determined. The numbers of exons and introns were identical to the human CYP17. It was suggested that the equine CYP17 gene exists as a single locus gene in a genome alike in case of humans, by accordance of the overlapping part of PCR clones include one or more introns. In the intron C of the equine CYP17 gene, two nucleotide sequences of 72 base-pairs homologous to 70 base-pairs core motif of the mammalian-wide interspersed repeats (MIRs) were tandemly repeated with Hind III restriction site between them.