Abstract
For a light microscopic examination from a large amount of horse hoof specimen, we usually apply a grinding technique using a hydrophobic resin to prepare a thin ground section. After preparation of the resin-embedded thin ground section (RGS), we stain it without prior deresinization or heating in any stages of the staining procedures in order to intensify the stain outcome. This technique includes an advantage to prevent RGS from modification. We do not study, however, the stain characteristics of the RGSs of the hoof tissues which are made by the above procedures. Therefore, we compared the RGSs (n=11) with the standard paraffin-embedded sections (PSs) (n=11) with H.E. stain, Ayoub-Shklar method and Dane's method. The H.E. stained RGS did not essentially differ from the one of the PS, but two special stain methods showed different stain characteristics compared with the stain outcome of the PS. From these results, H.E. stain is an applicable stain technique for the non-deresinized thin ground sections of the horse hooves.
