Production and Specificities of Monoclonal Antibodies to Horse Blood Group Antigens

Abstract

Monoclonal antibodies (mAbs) to red cell antigens of horse were produced by the standard method. Seventeen kinds of mAb (NHA-1, NHA-19, I-1C12, I-3E3, NHA-2, NHA-3, I-1E3, I-1A3, NAH-20, NAH-26, NAH-18, TA-1, NH-23, YA-1, I-3F10, 5C8 and NH-15) to horse blood group antigens were established, and their specificities were determined by the comparison test of the International Society for Animal Genetics. Out of these mAbs, both NHA-3 (anti-A₂-dose) and I-1A3 (anti-Ax-dose) recognized antigens belonging to the A system. The titer of the NHA-3 varied with the presence or absence of Aa and Ab antigens, whereas that of the I-1A3 varied with the presence or absence of Aa and Ac antigens. The NH-15 (anti-Px-dose) recognized antigens of the P system, and its titer varied with the presence or absence of the Pc antigen of this system. The quantitative antigen, recognized by the NAH-18 (anti-Ca-dose) belonging to the C system, was found to occur in larger quantities on the red blood cells in heavy breeds than on those in light breeds. The present study showed that these mAbs prepared to horse blood group antigens may be useful for more detailed analysis of antigenic subtype relationships and quantitative antigens.