Journal of Equine Science
Online ISSN : 1347-7501
Print ISSN : 1340-3516
ISSN-L : 1340-3516
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Effects of Percoll Washing and Glycosaminoglycans on the Lysophosphatidylcholin Induced Acrosomal Reaction in Stallion
Mikiko KOBAYASHIMissaka P.B. WIJAYAGUNAWARDANEYoung H. CHOIAna Yimiko KOJIMAShinichi HOCHINorihiko OGURIMitsuhiro TAKAGIKiyoshi MIYAZAWAKunitada SATO
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1998 Volume 9 Issue 4 Pages 101-106

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Abstract

The present study was conducted to investigate the effects of skim milk glucose (SMG), percoll and Brackett and Oliphant medium supplemented with BSA (BO-BSA) washing and glycosaminoglycans treatments (GAG) on lysophosphatidylcholin (LPC)-induced acrosome reaction (AR) in stallion spermatozoa. Freshly collected semen was washed with SMG (300 g for 10 min), percoll (700 g for 30 min) and BO-BSA (300 g for 10 min). Part of the semen after each washing step was treated with LPC to asses the rate of AR. After SMG, percoll and BO-BSA washing, the spermatozoa were cultured with in 5 different concentrations of GAG (chondroitin sulfate; CS and heparin; 0, 3, 10, 30, 100 μg/ml) for 0, 1.5, 3 and 6 h at 38.5°C in 5% CO2 in air. The LPC-induced AR in fresh spermatozoa after washing with SMG, percoll and BO-BSA were 0.8%, 3.0%, 13.4% and 18.0%, respectively. A significant difference in the rate of AR was observed before and after percoll washing (P<0.05). The LPC-induced AR rates after CS and heparin treatments were 14.7-20.8% and 15.3-19.5%, respectively. No relationship was observed either with the concentration of GAG or culture time on the percentage of AR spermatozoa. Results of the present study indicate that the percoll and BO-BSA washing can potentiate the LPC induced AR in stallion spermatozoa. Moreover, CS or heparin may not be useful in inducing AR in stallion spermatozoa.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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