Abstract
For research on hemolysis with exercise, changes of plasma amino acids and inorganic ions concentrations with maximum exercise (913±6.5 m/min) in 2 years old thoroughbred horses were investigated. Sixteen experimental horses were classified into two types by variation of free amino acid concentrations in red cells, namely basic amino acid-positive (BA+) type (3 females and 2 males) and -negative (BA-) type (5 females and 6 males). Blood samples were collected at 30 minutes before and 5 minutes after exercise. The results obtained are as follows; 1) MCHC slightly decreased after exercise. In contrast, osmotic fragility of red cell membrane and hemoglobin concentration in plasma significantly increased after exercise. 2) Concen trations of alanine, serine, threonine and lysine in plasma of BA+ type were similar to those of BA- type before exercise. However, the concentration of those amino acids in plasma of BA+ type significantly increased after exercise. 3) K+ concentration in plasma significantly increased after exercise in both BA types. The increase rate by exercise of K+ of BA+ type was significantly lower than that of BA- type. These results suggested that several substances are released from red cells to plasma be caused by hemolysis with maximum exercise.
