Abstract
A method for grinding thin sections o f resin-embedded hooves for histological analysis is described. Ten percent neutral formalin fixed material is dehydrated in a graded ethanol series, then embedded in resin. After polymerization, sections are cut to a width of 0.5-1.0 mm with diamond blade saw, and then ground to a thickness of 30-40 μm. The ground sections thus obtained are considered to be suitable for histological study of hooves.