Journal of Equine Science
Online ISSN : 1347-7501
Print ISSN : 1340-3516
ISSN-L : 1340-3516
Preparation of Thin Ground Section of Resin-Embedded Hooves for Histologic Analysis
Atsutoshi KUWANO
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JOURNAL FREE ACCESS

1994 Volume 5 Issue 1 Pages 37-39

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Abstract
A method for grinding thin sections o f resin-embedded hooves for histological analysis is described. Ten percent neutral formalin fixed material is dehydrated in a graded ethanol series, then embedded in resin. After polymerization, sections are cut to a width of 0.5-1.0 mm with diamond blade saw, and then ground to a thickness of 30-40 μm. The ground sections thus obtained are considered to be suitable for histological study of hooves.
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© Japanese Society of Equine Science
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