A Novel A-Kinase Anchoring Protein in the Heart Interacts with G α 13

Abstract

A cDNA of a tentative A-kinase anchoring protein, presumably coupled with heterotrimeric GTP binding protein α 13 subunit (G α 13), was cloned from a human heart cDNA library. It was approximately 650 bases and its mRNA was expressed in the heart. Homology search of DNA sequences revealed that it was a novel cDNA with 84% homology with the partial sequence of rabbit cDNA of AKAP 120 without a stop codon. 3'-Rapid Amplification of cDNA Ends (3'-RACE) and yeast functional assay were performed to determine the 3'-end of the cDNA and ribosomal frameshifting was suggested as a translational mechanism. Here we report that a protein encoded by the cDNA may be involved in intracellular signal transduction via the G α 13 and PKA in hearts.