Initial Calcification of Bone Formation in Ectopic and Osteogenic Tissue Induced by BMP-Collagen Composites

Abstract

In this study, the initial osteoinductive capacity and bone forming patterns induced by BMP were studied using routine histology and electron microscopy. The pattern of initial calcification is different in induced bone formation at heterotopic sites. Three experiments were conducted; BMP and atelocollagen complexes were implanted into subcutaneous and subperiosteal tissues of rats. Initial calcification multi potentially started in matrix vesicles of osteoid tissue as well as in carrier collagen fibers. Bone formation occurs either via preexisting cartilage or directly according to the geometry of carriers used and the micro environmental conditions. In this study we conducted an ultra structural analysis of subcutaneously implanted both bovine extracted BMP and rhBMP with collagen carriers and subperiosteally implanted rhBMP-collagen composites in rats to understand the effects of carrier and environmental conditions on bone formation.