Hydrogen Peroxide Reduced Osteomodulin Gene Expression in MC3T3-E1

Abstract

Bone formation steadily declines with age resulting in significant loss of bone mass, and reactive oxygen species (ROS) are thought to be a major contributor to the aging process. H₂O₂ treatment significantly reduced bone nodule formation rate in pre-osteoblastic cell, MC3T3E1. In this study, to identify which genes have an altered transcription level associated with bone loss by ageing, MC3T3-E1 cells were treated with H₂O₂, and monitored gene expression change using the Affymetrix GeneChip^TM system (Mouse; 34,000 genes). H₂O₂ altered many gene expressions including reduction of osteomodulin (OMD) mRNA level. The reduction of OMD gene expression by H₂O₂ was successfully confirmed by reversed transcription polymerase chain reaction (RT-PCR) and real-time PCR. Since OMD was found in bone tissues and play an important role in biomineralization, the reduction of OMD gene expression by H₂O₂ may be involved in the decline of bone formation in the ageing process.