Abstract
The purpose of the current research is to elucidate of gene expression of the tooth germ. Fresh permanent incisor and molar teeth germs were extracted from mandibles of 6-month-old pig. Extracted human premolars for orthodontic reasons were used as sample of the completed tooth root. Apical and central samples were prepared from pulp tissue. mRNA expression by RT-PCR and protein expression of BMP in the pulp tissue were detected. DMP1, BMP2 expressions were similar in all samples. OP expression was weaker in centrum of the molar tooth as compared to the other parts. OC was not expressed. BMP4 expression was strong at the incisor apical part, though it was not expressed at the molar central area. BMP5 was weakly expressed at the incisor central and molar apical areas. Though BMP7 expression was remarkably detected at the incisor and molar apexes, its expression was almost absent at the central area. Moreover for examining the specific expression of BMP7 at the apex, central superficial layer corresponding to the neck of the incisor and molar teeth was prepared. The result was that BMP7 was strongly expressed at the apex than in the central superficial layer and only a little expression was detected at central superficial layer of the incisor and molar tooth. In the current research, expressions of OP, DMP, BMP 2, 4, 5, 7 were seen in the tooth germ tissue. Thus the results suggested that expression of these genes played a central role in differentiation of the pulp. BMP7 was also suggested to have a specific role in apex formation (differentiation).
