Abstract
To observe the effect of bone formation with Bio-Oss guided by bioresorbable collagen membrane fixing. Four dogs were used in the study. Three 1cm×1cm areas were formed on bilateral mandibles randomly. The experiment include three groups: group A (Bio-OssR); group B (Bio-OssR + collagen membrane + titanium screws fixing); and group C (Bio-OssR + collagen membrane without fixing). The macroscopic, histological and scanning electron microscope observations were performed at 12 weeks postoperation. The percentage of newly-formed bone area was obtained by computer image analysis (Image-Pro Plus Computer Graphic Analysis System). The three groups all formed new bones. Group B: mature osteocytes and Haversian lamellar could be seen, and plenty of bone trabeculae were arranged in an orderly way. Group A: massive osteoblasts and bone trabeculae formation were found. Group C: there were many osteoblasts and bone lacuna, and osteocytes maturity was higher than that of group A. The average area percentage of new bone in group A was less than in groups B and C, and the average area percentage of new bone in group B was the highest (compared with groups A and C, P<0.05). These data suggest that the bone formation effectiveness of Bio-OssR combined with collagen membrane fixing was better than that of Bio-OssR only and collagen membrane without fixing.
