Abstract
The influence of high glucose on proliferation and expression of correlation factors including TNF related apoptosis inducing ligand (TRAIL), osteoprotegerin (OPG) and osteoprotegerin ligand (OPGL) of MG63 osteoblasts was detected in the present study. The relationship between the high concentration blood glucose and the physiological functions of human osteoblasts was studied. Then the pathogenesis of diabetes mellitus with chronic periodontitis was discussed. MG63 cells were cultured in different concentrations (5.5 mmol/l, 8 mmol/l, 11 mmol/l, 20 mmol/l, 30 mmol/l) medium for 24 hours. Cell proliferation was detected by MTT measurement. Flow cytometry was used to detect cell cycles. The mRNA expressions of TRAIL, OPG, and OPGL were detected by reverse transcription polymerase chain reaction (RT-PCR). The results showed that a low concentration of glucose has no significant influence on the proliferation of MG63 cells and other biological activity. With the increase of glucose concentration, the proliferation of MG63 cells was inhibited. The percentage of G1 phase cells was increased, the proportion of S phase kept unchanged and the percentage of G2 phase cells was decreased. With the increase of glucose concentration, the mRNA expressions of TRAIL and OPGL of MG63 cells showed an increasing trend while OPG showed an opposite trend. In conclusion, high glucose can inhibit the proliferation of MG63 and lead to the increasing mRNA expression of TRAIL and OPGL of human osteoblasts. However, the mRNA expression of OPG of human osteoblasts was decreased.
