Construction and Characterization of Three-Dimensional Silk Fibroin-Gelatin Scaffolds

Abstract

The objective of the study was to discuss the construction methods, characterization and biocompatibility of silk fibroin-gelatin (SF-G) three-dimensional scaffolds which meet the requirements of bone tissue engineering scaffolds. Silk fibroin (SF) and gelatin (G) were lyophilized at different temperature such as -20 °C, -40 °C, -60 °C, -80 °C then to make the composite scaffold materials crosslinked by genipin and methanol. Then to make composite materials, in order to find out the different temperature and crosslinking agent of SF and G, the following indexes including pore size, porosity, the water absorption and determination of mechanical properties were determined. The most appropriate condition of making SF-G scaffolds was pre-freezing temperature is -40 °C and cross-linked by genipin, which could meet the physicochemical requirements of bone tissue engineering.