Cloning of the Parathyroid Hormone Receptor in Japanese Quail

Abstract

Parathyroid hormone/parathyroid hormone-related protein receptor (PTH/PTHrP type 1 receptor; commonly known as PTH1R) is a family BG protein-coupled receptor that is mainly expressed in the bone and kidney of humans where it regulates skeletal development, bone turnover, and mineral ion homeostasis. The medullary bone of female birds is formed and resorbed in the presence of estrogen and androgen, while the administration of human estrogen to male Japanese quail results in artificial formation of the medullary bone in the bone marrow cavity. Therefore, male Japanese quail medullary bone is a good model for the study of bone homeostasis, and Japanese quail PTHR may play an important role in bone metabolism. However, the expression and function of PTHR in Japanese quail are unknown, and PTHR cloning is incomplete. Therefore, in this study, we attempted to clone the PTHR cDNA of Japanese quail.