Journal of Hard Tissue Biology
Online ISSN : 1880-828X
Print ISSN : 1341-7649
ISSN-L : 1341-7649
Evaluation of Guided Bone Regeneration Using the Bone Substitute Bio-Oss® and a Collagen Membrane in a Rat Cranial Bone Defect Model
Shin KasuyaShihoko InuiNahoko Kato-KogoeMichi OmoriKayoko YamamotoKazuya InoueYuichi ItoYoichiro NakajimaAzumi HirataTakaaki Ueno
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2018 Volume 27 Issue 1 Pages 79-84


The value of using the xenogeneic bone substitute Bio-Oss® and a collagen membrane for guided bone regeneration (GBR) was evaluated. Five-millimeter bone defects were created in the cranial bones of 15, 15-week-old, male Sprague-Dawley rats, five of which were left unfilled (Group A), five were filled with Bio-Oss® (Group B), and five were filled with Bio-Oss® and a collagen membrane (Group C). They were evaluated by micro-computed tomography (micro-CT) Weeks 8 after bone defect creation. The animals were euthanized in Week 8, and tissue samples were taken. The sampled tissue was evaluated by the Kawamoto technique for preparing nondecalcified frozen sections. On micro-CT, radiopacities in the bone defects were evident in Groups B and C, but not in Group A. The amount of radiopacity tended to be greater in Group C than in Group B. Histologically, no new bone formation was observed in Group A. In Groups B and C, new bone formation around the Bio-Oss® was apparent and some osteoblastic cells were observed along the new bone. The Bio-Oss® was not absorbed up to Week 8, suggesting that it may be a potential bone regeneration scaffold. The combined use of a collagen membrane anchored the Bio-Oss® in close contact with the cranial bone, suggesting that it may help create a favorable environment for bone formation. The combination of Bio-Oss® and a collagen membrane in GBR may be useful for bone defect regeneration.

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