2018 Volume 27 Issue 3 Pages 209-212
Ozone is currently being considered as a possible oral antiseptic agent because it is strongly antimicrobial and does not induce microbial resistance. Here, we examined the effects of ozone exposure on the production of collagen type-1 and inflammatory cytokines in primary human gingival fibroblasts (HGFs) in vitro using enzyme-linked immunosorbent assays. In this study, we demonstrated that ozone ointment increased type 1 collagen production and hindered pro-inflammatory cytokine secretion from primary HGFs in vitro. HGFs were isolated from a 65-year-old patient who had undergone surgery due to chronic periodontitis. The cells were exposed to media with or without 0.05, 0.5 and 5 ppm ozone ointment for 24 hours 2 min. No cytotoxic effect of the ozone ointment was observed up to the concentration of 0.5 ppm, cell viability was attenuated at the dose of 5 ppm. When ozone ointment was used at the non-cytotoxic concentration of 0.5 ppm, it significantly enhanced type 1 collagen production by HGFs within for 24 hours. Secretion of the pro-inflammatory cytokines interleukin (IL)-6 and IL-8 by HGFs treated with lipopolysaccharide (LPS) decreased when ozone ointment was present in the medium. These results suggest that the therapeutic effect of ozone ointment against periodontal disease is partially due to modulation of the function of HGFs.
