Abstract
Toluidine blue is metachromatic dye belonging to the thiazine group. Toluidine blue staining has been used for detection of exact area of carcinomatous invasion. The cases for this study related to the period from 1974 to 1978, during which a total of 151 microlaryngoscopies were performed by the same doctors group.
Though the 64 specimens seeming to be non-malignant under simple micolaryngoscopic view, their histological features exposed 50 non-malignant specimens (78%), but 14 specimens (22%) of malignant histological reports.
Out of 87 specimens looked like malignant tissues under microlaryngoscopic view, their histological features showed the 69 (79%) malignant results, but the 18 (21%) specimens of non-nalignancy. Agreement of the histological-microlaryngoscopic views was shown at the rate of 78.8% (119/151).
On the other hand, histological features exposed 59 (84%) malignancy though the 70 specimens seeming to be malignant by positive staining reaction with toluidine blue.
Out of 48 specimens reacted to be negative by toluidine blue staining, their histological features showed the 48 (100%) benign results. Agreement of the histological-microlaryngoscopic views was shown at the rate of 90.7% (107/118). Histological features of the giving positive staining reaction, no matter how their outlooking with an aid in the microlaryngoscopic views seeming normal lesions, included 3 specimens of carcinomas in situ and 1 specimen of severe dysplasia.
Toluidine blue seemed to be useful for detection of carcinoma in situ, determination of the lesion of biopsy, detection of multifocal carcinoma and adequate choice of treatment of laryngeal cancer.
