Abstract
We report here a useful method of immune elimination of nude mouse stromal cells for the cultivation of human tumors transplanted into nude mice. We used the rabbit antiserum immunized with spleen cells obtained from nude mice and a normal rabbit serum as the source of complement. Complement-dependent cytotoxicity was determined by direct cytotoxic test. The antiserum in the presence of complement specifically killed cultured cells derived from nude mice, but it was not cytotoxic to cultured cells drived from human. When cultures consisting of human cells and nude mouse cells were treated with the antiserum and complement, the nude mouse cells were selectively removed from the culture and this was determined by fluorescent antibody technique.
