The Circulating Interferon (IFN) Assey of the Sudden Deafness Patients

Abstract

Viral infection is believed to be one of the causes of sudden deafness. The circulating interferon (IFN) assey can detect viral infection at early stage.
The circulating interferon (IFN) of 31 patients of sudden deafness (41 samples) were asseyed by means of 50% cell pathological effect (CPE) reduction method in microplate using FL cell-Vesicular Stomatitis Virus (VSV) system.
The samples were obtained within a few days from the onset of the hearing loss.(Twenty-seven were within a week.) No interferon activity was detected in the patient serums. At the same time, the detection of the virus particle from serum of the patient, by CPE formation in FL cells and Vero cells, was performed. No CPE was observed after 7 days in FL cells, and 10 days in Vero cells from the innoculation. This work did not indicate the relationship between viral infection and sudden deafness.