Abstract
To demonstrate endogenous androgen in normal human laryngeal mucosa, carcinoma and certain other lesions, 6 normal male epitheriums, 8 carcinomas, 10 polyps, hyperplasias and 4 heteromorphic epitherium tissues were processed for the histochemical demonstration of endogenous androgen by using the ABC immunohistochemical technique. Every carcinoma used in this study were moderately differentiated squemous cell carcinomas. The ABC method demonstrated a positive reaction in the nuclei of two male laryngeal carcinoma tissues and the laryngeal muscle. In the staining protocol employed, the other tissues gave negative reactions. The result showing positive reactions in nuclei were similar to those demonstrated by other sex hormone target organs in the literature. These results produced some evidence to suggest that human laryngeal carcinoma might be a target for androgenic steroids, and therefore implies that androgen plays an important role in the carcinogenetic process of normal laryngeal tissue and, for example, on the development and growth of laryngeal carcinoma.
