Abstract
Chemotherapy is an important therapy of head and neck carcinomas. The emergence of chemotherapeutic drug resistance is a major clinical problem and the reason for failure in the treatment of patients with head and neck cancer. It is very important to predict the response of chemotherapy of head and neck cancer. We reported two methods of anticancer drug sensitivity test. SDI test is an in vitro test, based on the correlation of succinate dehydrogenase activity using tetrazolium salt as a hydrogen acceptor, with cell viability. This method's good points are as follows: 1: It is very simple and could be able to deal many samples at a time. 2: It takes only short time to do and it has good reappearance. 3: It could be able to elect ineffective drugs. This method's weak points are as follows: 1: It has an aspect loss of interaction of cells because this method uses only malignant cells viabillity. 2: It has low rate of judgment. 3: There are low relationship between this result and clinical course. 4: There are influence of intermediate cells. HDRA method is reported by Freeman and Hoffman at 1986. This method's good points are as follows: 1: Measurement is done by preserving the interaction of cell. 2: Rate of judgment is higher than SDI method. 3: Correlation to clinical course is high. This method's weak points are as follows: 1: Measurement time is long. We reported the relationship between p53 expression and response of chemotherapy. The negative cases of p53 had a tendency of good response of chemotherapy, but positive cases of p53 had a tendency of bad response. We concluded that assessment of p53 protein expression would be able to predict the response of chemotherapy. Then we may be able to elect the effective drugs using HDRA method and inffective drugs using SDI method.
