Flow Cytometric DNA Analysis and MR Imaging of Salivary Gland Tumors

Arata Horii; Jun-ichi Yoshida; Yuichiro Honjo; Kenji Mitani; Kenji Hattori; Takeshi Kubo; Masahide Sakai; Shigeru Okamoto; Shodayu Takashima

doi:10.3950/jibiinkoka.101.289

Arata Horii, Jun-ichi Yoshida, Yuichiro Honjo, Kenji Mitani, Kenji Hattori, Takeshi Kubo, Masahide Sakai, Shigeru Okamoto, Shodayu Takashima

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Keywords: salivary gland tumor, MR images, flow cytometry, cell cycle, pre-operative diagnosis

JOURNAL FREE ACCESS

1998 Volume 101 Issue 3 Pages 289-296

DOI https://doi.org/10.3950/jibiinkoka.101.289

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Published: March 20, 1998 Received: - Available on J-STAGE: March 19, 2008 Accepted: - Advance online publication: - Revised: -
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Date of correction: March 19, 2008 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: March 19, 2008 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: March 19, 2008 Reason for correction: - Correction: TITLE Details: Right : Flow Cytometric DNA Analysis and MR Imaging of Salivary Gland Tumors

Date of correction: March 19, 2008 Reason for correction: - Correction: ABSTRACT Details: Right : We correlated the histopathology of 32 cases of salivary gland tumors with MR imaging and flow cytometric DNA analysis. All malignant tumors were my isivasive and/or had an ill-defined margin. Fifty-seven percent of the pleomorphic adenomas were shown as a high signal intensity area on T2 weighted images. Fifty-six percent of Warthin tumors were shown as a low or iso signal intensity area on T2 weighted images associated with the cystic portion. Sixty seven percent of malignant tumors were characterized by DNA aneuploidy and/or a total percent age of the S phase fraction plus the G2+M fraction higher than 10%. It was less than 10% and the Ki-67 positive fraction was higher than 20% in all pleomorphic adenomas. The Ki-67 positive fraction was less than 20% in 78% of Warthin tumors. The histopathology of all malignant tumors, 86% of the pleornorphic adenomas, and 89% of Warthin tumors was correctly predicted by the combination of MR images and flow cytometry. We suggest that the MR images combined with flow cytometric analysis of fine needle aspiration-derived materials is useful in the pre operative histopathologic diagnosis of salivary gland tumors.

Date of correction: March 19, 2008 Reason for correction: - Correction: AUTHOR Details: Right : Arata Horii¹⁾, Jun-ichi Yoshida²⁾, Yuichiro Honjo²⁾, Kenji Mitani²⁾, Kenji Hattori¹⁾, Takeshi Kubo¹⁾, Masahide Sakai³⁾, Shigeru Okamoto³⁾, Shodayu Takashima⁴⁾

Date of correction: March 19, 2008 Reason for correction: - Correction: AFFILIATION Details: Right :
1) Department of Otolaryngology, Osaka University Medical School
2) Department of Otolaryngology. Osaka Teishin Hospital
3) Department of Pathology, Osaka Teishin Hospital
4) Department of Radiology, Shinshu University School of Medicine

Date of correction: March 19, 2008 Reason for correction: - Correction: KEYWORD Details: Right : salivary gland tumor, MR images, flow cytometry, cell cycle, pre-operative diagnosis

Date of correction: March 19, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

We correlated the histopathology of 32 cases of salivary gland tumors with MR imaging and flow cytometric DNA analysis. All malignant tumors were my isivasive and/or had an ill-defined margin. Fifty-seven percent of the pleomorphic adenomas were shown as a high signal intensity area on T2 weighted images. Fifty-six percent of Warthin tumors were shown as a low or iso signal intensity area on T2 weighted images associated with the cystic portion. Sixty seven percent of malignant tumors were characterized by DNA aneuploidy and/or a total percent age of the S phase fraction plus the G2+M fraction higher than 10%. It was less than 10% and the Ki-67 positive fraction was higher than 20% in all pleomorphic adenomas. The Ki-67 positive fraction was less than 20% in 78% of Warthin tumors. The histopathology of all malignant tumors, 86% of the pleornorphic adenomas, and 89% of Warthin tumors was correctly predicted by the combination of MR images and flow cytometry. We suggest that the MR images combined with flow cytometric analysis of fine needle aspiration-derived materials is useful in the pre operative histopathologic diagnosis of salivary gland tumors.

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