A STUDY ON THE OXYGENCONSUMPTION AND FUNCTION OF THE STRIA VASCULARIS AND THE SPIRAL LIGAMENT

Abstract

In order to obtain some grounds for interpretation for the origin of the inner ear disease, the cochlea response, physiology of hearing mechanism and to aid the pharmacological treatment of the inner ear deafness, the oxygen consumption of the stria vascularis and the spiral ligament was studied.
The function of the stria vrscularis and the scopy.
Healthy adult guinea pigs were used in the series of the experiments.
Results obtained were as follows: 1. First of all by use of the cartesian diver cularis with the spiral ligament and the effect of Krebs Ringer Phosphate selution, glucose, amino acid Krebs Ringer Phosphate solution, and diffecnce of pH, and every turn and individual stria vascularis and the spiral ligament were studied.
a) Oxygen consumption of the materials was phate solution.
b) In 0.2% glucose Krebs Ringer Phosphate solution, the velosity of the oxygen consumption showed a steady rate and its rate was twice that of K.R.P. solution. maximum and decreased at pH under pH 7.0 or over pH 7.4.
d) When glutamic acid, aspartic acid, alanine was added in K.R.P. solution, great increased in the oxygen consumption was indicated and a raise in the oxygen consumption also comes after adding glycine, threonine, and valin, but the addition of ase in the rate.
e) When the oxygen consumption of every turn was taken in 0.2% glucose K.R.P. solution, the second turn showed the most in quantity and decrease in the following desending order, third turn, forth turn and basal turn, there by showing that the metabolism is differs in every turn.
ment was also large.
2. Influence of Dihydrostreptmcin (D.H.S.M).
1) The oxygen consumption of the materials dectreased when D.H.S.M. was injected in guinea pig, in vivo.
2) Both in vivo and in vitro, when added to the medium or injected Vitamin B1, decreased the obstruction of the oxygen consumption by D.H.S.M.
3) In vivo, when thioctic acid was injected together with D.H.S.M., the obstructed degree of the oxygen consumption is decreased, but no change was seen, when a little amount of thiotic acid was added to the medium with D.H.S.M. Hwever, the oxygen consumption decreased when a large quantity was added to the medium.
4) Both in vivo and in vitro, when TATD was added, the obstructed degree of the oxygen consumption by D.H.S.M. is decreased.
5) When the liver was injured with injection of CC14 to guinea pigs, the oxygen consumption of the stria vascularis and the spiral ligament showed no change, but in these cases, the influense of D.H.S.M. is larger than in healthy animals.
6) The oxygen consumption of every turn in 0.2% glucose K.R.P. solution with added D.H.S.M. was reduced in the following descending order, basal turn, forth turn, second turn, third turn.
3. As a tracer, 5% dextran iron solution was administered to guinea pigs and followed by electron microscopy, and the results obtained were as follows.
1) When the dextran iron solution was injected lary of the stria vascularis and the spiral ligament lary as pinocytic vesicules but after twenty four hours, the tracer could not be found in the cells, ranes was presumed.
2) Detxtran iron solution was injected into the endolymphaic space. The tracer introduced into the spiral ligamet's cells at external sulcus and detailed form of movement in cells and intercellular spaces was observed.
3) Dextran iron solution was injected into the perilymphatic space. The tracer introduced into the spiral ligament and the detailed forms of movement in cells and intercellular spaces could be seen.