EXPERIMENTAL STUDY ON ALLERGIC OTITIS MEDIA

Part 2: Allergenic Effect of Bacterial Component of Proteus Morgani

Abstract

The bacterial components A and B were extrated from Proteus morgani by a special method. The bacterial component A was weak in effect of protein and strong in effect of lipopolysaccharoid and the bacterial component B was strong in effect of protein and weak in effect of lipopolysaccharoid. The bacterial component A was regarded as carbohyderate fraction and the bacterial component B was regarded as protein fraction. The bacterial component of proteus morgani was separately injected into the bulla of the middle ear of the nonsensitized adult guinea pigs.
After single injection and also after two to six multiple injections with an interval of 10days, those animals were sacrificed and histological examination temporal bones of the were carried out.
In the animals receiving single injection, there were slight allergic findings but no significant differences among the animals received different experimental procedures.
In the animals receiving two to six multiple injections of bacterial component A or B.
There found goblet cell formation and flattening of the ciliary cells in the epithelial cells of the bullar mucosa.
In the submucous tissue, inflammatory finding was prominent and the degree of increase in plasma cells was paralleled to the number of injection.
In the labyrinth of all specimens receiving multiple injections, dilatation of the stria vascularis and vesicle formation in the epithelial cells were noticed.
Cells were mostly swollen in the organ of Corti, but Reissner's membrane was almost normal. Conclusively, the allergenic effect of bacterial components A and B was detectable by our experimental study, and the effect of B was more prominent than that of A.