LYMPHOCYTE PROLIFERATION ASSAY OF THE TUMOR ASSOCIATED ANTIGEN DERIVED FROM A NASOPHARYNGEAL CARCINOMA CELL LINE (NPC-204)

Abstract

Tumor associated antigen (TAA) of a nasopharyngeal carcinoma (NPC) cell line (NPC-204) was extracted and its specificity was examined by the lymphocyte proliferation assay in 40 Japanese and 12 Chinese NPC patients.
1. The simulation index was significantly high in 27 of the 40 Japanese and 7 of the 12 Chinese NPC patients.
2. PPD skin test and measurement of total lymphocyte count and the subpopulation of T and B lymphocytes were performed. These parameters showed no correlation with the stimulation index.
3. Tumor associated antigen of Raji cells was extracted and its specificity was examined by the same assay. The stimulation index was also significantly high in NPC patients.