ESTABLISHMENT OF A PERMANENT HUMAN MAXILLARY SINUS CARCINOMA CELL LINE AND GENERATION OF A MONOCLONAL ANTIBODY REACTIVE WITH MAXILLARY SINUS CARCINOMA

Abstract

A permanent cell line (AT-MS-1) has been established from a maxillary sinus squamous cell carinoma of the patient (K.I.) and the monoclonal antibodies against maxillary sinus carcinoma cells were generated using the AT-MS-1 cells. AT-MS-1 has been passaged 68 times during 26 months.
The doubling time of this cell lines is approximately 43 hours.
In nude mice, AT-MS-1 produced a growing tumor with distinct histological features of squamous cell carcinoma.
Antibodies-secreting hybridomas were generated by fusion of mouse myeloma cells with mouse spleen cells immunized with the AT-M-1 cells.
The supernatants of hybridomas were tested against a panel of aceton-alcohol fixed monolayer human cells by indirect immunofluorescence analysis and examined against T cell, B cell and myeloid cell lines using cellular radioimmunoassay.
The positive supernatants were further examined on tissue sections with immunoperoxidase technique.
The monoclonal antibody 10B2 reacted with maxillary sinus carinoma cells and did not react with stroma, infiltrating cells and mucous epithelium.
Monoclonal antibody 10B2 is a useful antibody to discriminate maxillary sinus carcinoma cells from nomal maxillary sinus cells.