TRANSFECTION OF HUMAN NASOPHARYNGEAL EPITHELIAL CELLS WITH EPSTEIN-BARR VIRUS DNA DERIVED FROM NASOPHARYNGEAL CARCINOMA

Abstract

Transfection experiments using Epstein-Barr virus (EBV) DNA obtained from NPC-KT cells were carried out to examine whether the Ad-AH human adenoid epithelial cells could be transfected. We found that the Ad-AH cells were transfectable using NPC-KT virus DNA, as determined by the synthesis of EBV specific antigens. Concentrates of supernatant fluids obtained from transfected Ad-AH cells were capable of transforming human cord blood lymphocytes. Similar virus concentrates were unable to superinfect Raji cells.