TISSUE CULTURE OF MIDDLE EAR EPITHELIUM OF THE GUINEA PIG DIFFERENCES OF THE CELLULAR GROWTH ACTIVITY IN THE MIDDLE EAR CAVITY USING COLLAGEN GEL CULTURE METHOD

SACHIO TAKENO

doi:10.3950/jibiinkoka.93.2038

TISSUE CULTURE OF MIDDLE EAR EPITHELIUM OF THE GUINEA PIG

DIFFERENCES OF THE CELLULAR GROWTH ACTIVITY IN THE MIDDLE EAR CAVITY USING COLLAGEN GEL CULTURE METHOD

SACHIO TAKENO

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JOURNAL FREE ACCESS

1990 Volume 93 Issue 12 Pages 2038-2046

DOI https://doi.org/10.3950/jibiinkoka.93.2038

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Published: July 20, 1990 Received: - Available on J-STAGE: March 19, 2008 Accepted: - Advance online publication: - Revised: -
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Date of correction: March 19, 2008 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: March 19, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : Primary tissue culture system from the middle ear and the tracheal epithelium of the guinea pig was established using collagen gel method. The cultured epithelium was studied by phase-con- trast microscopy, light and scanning electron microscopy. The outgrowthal area of the epithelium was quantitatively measured for period of up to ten days. In the middle ear cavity, the mucosal explants were sampled from various sites in order to compare their differentiational and prolifer- ative activities. The mucosal explants attached on collagen substrates were composed of ciliated, non-ciliated, goblet and basal cells. This basic structure was similar to the natural middle ear epithelium. The ciliated cells showed well organized cilia. Most of the outgrowthal cells devoid of fibroblastic cells in the monolayer were polygonal shaped with numerous microvilli. The morphology of the outgrowthal cells changed from columnar or cuboidal to squamous shapes in the area away from the explants. There was a correlation between the distribution of the ciliated cells in the outgrowths and these in the explants. The explants with columnar or cuboidal ciliated epithelia sampled from the opening of the eustachian tube or its neighborhood formed more than eightfold outgrowthal sheets in vitro. This is comparable to tracheal epithelia. On the other hand, the explants with simple squamous epithelia sampled from the area distal to the eustachian tube showed about fourfold proliferative activity. We concluded that this culture system would be useful for the study of cellular multiplication and differentiation mechanisms of the respiratory tract epithelium.
Right : Primary tissue culture system from the middle ear and the tracheal epithelium of the guinea pig was established using collagen gel method. The cultured epithelium was studied by phase-contrast microscopy, light and scanning electron microscopy. The outgrowthal area of the epithelium was quantitatively measured for period of up to ten days. In the middle ear cavity, the mucosal explants were sampled from various sites in order to compare their differentiational and proliferative activities.
The mucosal explants attached on collagen substrates were composed of ciliated, non-ciliated, goblet and basal cells. This basic structure was similar to the natural middle ear epithelium. The ciliated cells showed well organized cilia. Most of the outgrowthal cells devoid of fibroblastic cells in the monolayer were polygonal shaped with numerous microvilli. The morphology of the outgrowthal cells changed from columnar or cuboidal to squamous shapes in the area away from the explants. There was a correlation between the distribution of the ciliated cells in the outgrowths and these in the explants.
The explants with columnar or cuboidal ciliated epithelia sampled from the opening of the eustachian tube or its neighborhood formed more than eightfold outgrowthal sheets in vitro. This is comparable to tracheal epithelia. On the other hand, the explants with simple squamous epithelia sampled from the area distal to the eustachian tube showed about fourfold proliferative activity.
We concluded that this culture system would be useful for the study of cellular multiplication and differentiation mechanisms of the respiratory tract epithelium.

Date of correction: March 19, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

Primary tissue culture system from the middle ear and the tracheal epithelium of the guinea pig was established using collagen gel method. The cultured epithelium was studied by phase-contrast microscopy, light and scanning electron microscopy. The outgrowthal area of the epithelium was quantitatively measured for period of up to ten days. In the middle ear cavity, the mucosal explants were sampled from various sites in order to compare their differentiational and proliferative activities.
The mucosal explants attached on collagen substrates were composed of ciliated, non-ciliated, goblet and basal cells. This basic structure was similar to the natural middle ear epithelium. The ciliated cells showed well organized cilia. Most of the outgrowthal cells devoid of fibroblastic cells in the monolayer were polygonal shaped with numerous microvilli. The morphology of the outgrowthal cells changed from columnar or cuboidal to squamous shapes in the area away from the explants. There was a correlation between the distribution of the ciliated cells in the outgrowths and these in the explants.
The explants with columnar or cuboidal ciliated epithelia sampled from the opening of the eustachian tube or its neighborhood formed more than eightfold outgrowthal sheets in vitro. This is comparable to tracheal epithelia. On the other hand, the explants with simple squamous epithelia sampled from the area distal to the eustachian tube showed about fourfold proliferative activity.
We concluded that this culture system would be useful for the study of cellular multiplication and differentiation mechanisms of the respiratory tract epithelium.

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