Human Papillomavirus in Laryngeal Papilloma Detected by Dot Blot Hybridization (Vira Pap^TM) and Southern Blot Hybridization

Abstract

We demonstrated the presence of human papillomavirus (HPV) in 3 of 4 patients with laryngeal papillomas by dot blot hybridization (Vira Pap^TM) and Southern blot hybridization.
1. The HPV was type 6 in 2 patients and type 11 in 1 patient.
2. The results of this simple detection test (Vira Pap^TM kit) for dot blot hybridization correlated well with that of Southern blot hybridization. Therefore, this method is useful as a screening test.
3. Dot blot hybridization using serial dilutions of the homogenate of a HPV-positive papilloma revealed that 0.125mg wet weight of the papilloma sample was enough for HPV detection.