The Cell Organization of FDC Cluster in the Human Tonsils

Abstract

Follicular dendritic cells (FDC) are specialized cells found only within lymphoid follicles, and are significant factors in the humoral immuno system at the germinal center in the lymphatic tissue. However, evidence of the localization of FDC and other lymphatic cells in the human tonsil is lacking. We examined and analyzed the labelling pattern in the human tonsillar tissue produced by six monoclonal antibodies raised against DRC-1, PC OKT-4, OKT-8, Leu-7 and macrophages, and visualized using the avidin-biotin-peroxidase Complex (ABC) immunohistochemical technique.
FDC using the Anti-DRC antibody show e d nonstructural net formation in the whole germinal center, and the nuclei could not be stained. A positive figure was recognized in the cell membrane. Electronmicrographic evidence showed that FDCs extended their dendrites to the whole portion of the follicule and kept in contact with several distinct lymphocytes.