Examination of the Adherence of Streptococcus Pyogenes to Tonsillar Epithelial Cells Using a Flowcytometry Two Color Analysis

Abstract

We examined the adherence of Streptococcus Pyogenesto tonsillar epithelial cells, based on age and the presence or absence of a chronic infection, using a flowcytometry two-color analysis.
To determine the fibronectin on the tonsillar epithelial cells, we used the FITC-conjugated anti-fibronectin antibody, and to determine the bacterium, added avidin-PE to the biotinylated bacterium.
The number of bacteria adhering to epithelial cells decreased as a result of decreased fibronectin with the advance in age, and tonsillar epithelial cells from the patients with chronic infection contained more bacteria than those from normal healthy individuals. However, the patients with chronic infection are in remission.